Ⅱ QISM: G. Fruticosusning nefrolitik Wistar erkak kalamushlarida davolash samaradorligini baholash
Mar 22, 2022
Aloqa: Audrey Xu Whatsapp/hp: 0086 13880143964 Email:audrey.hu@wecistanche.com
Tilahun Alelign, Tesfaye Sisay Tessema, Asfaw Debella va Beyene Petros
Abstrakt
Ma'lumot: Efiopiya xalq tabobatida dorivor o'simliklar urolitiyozni davolashi mumkinligi haqida da'vo mavjud, ammo ilmiy dalillar etarli emas. Ushbu tadqiqotning maqsadi eksperimental induktsiya qilingan nefrolitiatik kalamushlarda Gomphocarpus fruticosus ekstraktining shifobaxsh samaradorligini baholash edi.
Usullari: urolitiyoz erkak Wistar kalamushlarda 28 kun davomida ichimlik suvida etilen glikol bilan oziqlantirish orqali qo'zg'atildi. Terapevtik ta'sir 15 dan 28 kungacha 200 mg / kg ekstraktlarni og'iz orqali yuborishdan keyin baholandi. Siydik namunalari kalamushlarni qurbon qilishdan 1 kun oldin olingan. 28-kuni anestetik holatda qon, jigar va buyrak namunalari yig'ildi. Siydikdagi kristallar yorug'lik mikroskopida ham tahlil qilindi.
Natijalar:G. fruticosusEtOAc ekstrakti natriy darajasini sezilarli darajada kamaytiradi (P<0.001), whereas="" it="" was="" significantly="" elevated="" the="" levels="" of="" magnesium="" and="" citrate=""><0.01)compared to="" lithiatic="" control.="">G. fruticosusBuOH ekstrakti kaliy darajasini pasaytirdi (P<0.01), calcium,="" and="" phosphate="" in="" urolithiatic="" rats.="" it="" was="" also="" observed="" that="">G. fruticosusEtOAc ekstrakti siydikdagi oksalat darajasini pasaytirdi (P<0.001), whereas="" it="" was="" increased="" the="" levels="" of="" magnesium=""><0.05) and="" citrate=""><0.01) in="" serum="" analysis="" after="" exposure="" to="" buoh="" extract.="" in="" the="" kidneys,="" caox="" crystal="" deposits="" were="" reduced="" significantly="" by="">G. fruticosusEtOAc ekstrakti (P<>
Xulosa: Ta'kidlanganidekG. fruticosusEtOAc ekstrakti urolitiyozni davolashda kuchli edi. Biroq, urolitiyozga qarshi faol birikmalarning samaradorligini baholash uchun qo'shimcha tadqiqotlar talab etiladi. Kalit so'zlar: Samaradorlik, Gomphocarpus fruticosus, In vivo, Urolitiyoz
Ⅰ Ⅱ QISM UCHUN SHU YERGA BOSING
Natijalar
G. fruticosus ekstraktlarining shifobaxsh samaradorligi
Terapevtik (terapevtik) tadqiqotlarda tanlangan ekstraktning dozasi kalamushlarning tana vazniga 2{6}}0 mg/kg ni tashkil etdi, bu maksimal ruxsat etilgan 2000 mg/kg bw dozaning o'ndan bir qismini tashkil etdi [32]. Bu 2000 mg/kg dozagacha uning xavfsizligini ko'rsatuvchi oldingi o'tkir va/yoki subakut toksiklik tadqiqotlari asosida tanlangan. Ichimlik suvida ammoniy xlorid (1 foiz) bilan birgalikda 0,75 foiz EG qo'llash orqali buyrak toshlarining paydo bo'lishi Wistar erkak kalamushlarida tasdiqlangan.
Siydikni (24 soat) fotomikroskopik tahlil qilish
Siydik namunalarini tahlil qilish kristall zichligi va o'lchamidagi o'zgarishlarni ko'rsatdi. OrasidaG. fruticosusketma-ket erituvchi ekstraktlari,G. fruticosusEtOAc ekstraktlari (1k-rasm)vaG. fruticosusBuOH ekstraktlari (1-rasm) kristall soni va o'lchamlarini ham kamaytirdi (1-rasm).
1-rasm. 200 mg/kg ekstrakt dozasida davolovchi davolashdan keyingi CaOx kristallarining siydik fotomikrograflari.Kaltsiy oksalat kristalining morfologiyasi va yorug'lik mikroskopi ostida ko'rilgan soni (40x), Erkak Wistar kalamushlarining ertalabki siydigi a Oddiy nazorat/avtomobil; b Litiatik nazorat; c kaliy sitrat (K-Cit) bilan davolash; d siston; IG. fruticosusPET ekstrakti; jG. fruticosusChl. ekstrakti; kG. fruticosusEtOAc ekstrakti; lG. fruticosusBuOH ekstrakti; va mG. fruticosussuvli fraktsiya.
Elektrolitlar va buyraklar faoliyatini tekshirish uchun siydik va sarum tahlili
Eksperimental kalamushlarda,G. fruticosusEtOAc ekstrakti litiatik nazorat bilan solishtirganda natriy darajasini sezilarli darajada kamaytirdi (P<0.001). the="" level="" of="" potassium="" was="" reduced="" by="">G. fruticosusBuOH ekstrakti (P<0.010). treatment="" with="" the="" aqueous="" extract="" of="" etoac="" and="" buoh="" extracts="" of="">G. fruticosusSiydik bilan oqsillar ajralishining sezilarli darajada kamayganligini ko'rsatdi (P<0.011) in="" relation="" to="" lithiatic="" control.="" sodium="" concentration="" was="" also="" reduced="" very="" significantly="" with="" the="" treatment="" of="" etoac="" extract="" of="">G. fruticosus (P<0.001). treatment="" by="" the="" extract="" of="">G. fruticosusBuOH litiatik guruhga nisbatan siydik kislotasi darajasini pasaytirdi (P<0.050). however,="" lithogenic="" induction="" may="" cause="" impairments="" of="" renal="" function="" as="" evidenced="" by="" raising="" the="" levels="" of="" creatinine,="" proteins,="" and="" uric="" acids.="" in="" the="" serum="" analysis,="" the="" level="" of="" potassium="" was="" reduced="" by="">G. fruticosusLitiatik nazorat bilan solishtirganda EtOAc ekstrakti (P<0.010). it="" was="" also="" found="" that="" the="" effects="" of="">G. fruticosusXlorid darajasi bo'yicha BuOH ekstrakti normal nazoratga yaqin edi. Kreatinin darajasi ko'tarildiG. fruticosusxom ekstrakti va PET ekstrakti (P<0.01)compared to="" the="" normal="" control="" (table="">
tsitanx tubulosa
2-jadval. Davolanishdan keyingi 28-kuni 200 mg/kg dozada tanlangan o'simlik ekstraktlari bilan davolangan eksperimental erkak Wistar kalamushlarida buyrak toshlarini hosil qiluvchi elektrolitlar va buyrak funktsiyasi belgilarining siydik (24 soat) chiqarilishi va sarum tahlili.Aralash ekstrakt {0}} A. Aspera, S. punctata va R. abyssinicus ekstraktlari (1:1:1 nisbat), suvli (suv), etil asetat (EtOAc), butanol birikmasi (BuOH), Xloroform (Chl) va neft efiri (PET). Ma'lumotlar har bir guruhdagi 6 ta kalamush uchun o'rtacha ± SD taqdim etildi (n=6). Vositalar o'rtasidagi taqqoslash I guruh (avtomobilni boshqarish) va II guruh (litiatik nazorat) bilan taqqoslandi. *p < 0.{{10}}5,="" **p="">< 0.01,="" ***p="">< 0,001="" nisbatan="" sezilarli="" o‘zgarishlarni="" ko‘rsatadi.="" i="" guruh="" (avtomobilni="" boshqarish),="" #p=""><0,05, ##p=""><0,01, ###p=""><0,001 ii="" guruh="" (litiatik="" nazorat)="" bilan="" solishtirganda="" sezilarli="" o'zgarishlarni="">
Kristal hosil bo'lish ingibitorlari va promotorlarining siydik va sarum tahlili
3-jadvalda ko'rsatilganidek, magniy darajasi qo'shimchalar bilan sezilarli darajada oshdiG. fruticosusLitiatik nazorat bilan solishtirganda EtOAc ekstrakti (P<0.010). similar="" to="" k-cit,="" the="" effects="" of="">G. fruticosusEtOAc ekstrakti sitrat darajasini sezilarli darajada oshirdi (P<>G. fruticosusBuOH ekstrakti siydikdagi kaltsiy va fosfatning yuqori darajasini kasal (litiatik) nazorat (I guruh kalamushlari) bilan solishtirganda sezilarli darajada pasaytirdi.<0.010). it="" was="" also="" observed="" that="">G.fruticosusEtOAc ekstrakti litiatik nazorat bilan solishtirganda rindagi oksalat darajasini pasaytirdi (P).<0.001). in="" the="" serum="" analysis,="" the="" excretions="" of="" calcium,="" oxalate,="" and="" phosphate="" were="" grossly="" increased="" in="" lithiatic="" induced="" male="" wistar="" rats.="" the="" etoac="" extract="" of="">G. fruticosusmagniy kontsentratsiyasini oshirdi (P<0.052). the="" serum="" level="" of="" citrate="" was="" increased="" after="" exposure="" to="" buoh="" extract="" of="">G. fruticosus(P<0.011), suggesting="" similar="" effects="" to="" potassium=""><0.001).>G. fruticosusChl. ekstrakti odatdagi nazorat bilan solishtirganda sitrat darajasini sezilarli darajada pasaytirdi (P<0.001)(table>
3-jadval. 200 mg/kg dozadagi o'simlik ekstraktlarining davolashdan keyingi 28-kunida erkak Wistar kalamushlarida siydik chiqarish (24 soat) va sarum kristall ingibitorlari va promotorlaridagi o'zgarishlarga ta'siri.Aralash ekstrakt {0}} A. aspera, S. punctata va R. abyssinicus ekstraktlarining kombinatsiyasi (1: 1:1 nisbat). Ma'lumotlar har bir guruhdagi 6 ta kalamush uchun o'rtacha ± SD sifatida taqdim etildi (n=6). Vositalar o'rtasidagi taqqoslash I guruh (avtomobilni boshqarish) va II guruh (litiatik nazorat) bilan taqqoslandi. *p < 0.{{10}}5,="" **p="">< 0.01,***p="">< 0.001="" nisbatan="" sezilarli="" oʻzgarishlarni="" koʻrsatadi.="" guruh-i="" (avtomobilni="" boshqarish),="" #p=""><0,05, ##p=""><0,01, ###p=""><0,001 ii="" guruh="" (litiatik="" nazorat)="" bilan="" solishtirganda="" sezilarli="" o'zgarishlarni="">
Litiatik nazoratda alanin aminotransferaza (ALT) va aspartat aminotransferaza (AST) ning sarum darajasi normal (sog'lom) nazoratga (P) nisbatan oshdi.<0.050).>G. fruticosusPET ekstrakti sarum AST darajasini sezilarli darajada oshirdi (P<0.001)compared to="" the="" healthy="" control.="" buoh="" extract="" of="" g.fruticosus="" lowered="" significantly="" the="" level="" of="" ast="" similar="" to=""><0.001) compared="" to="" lithiatic="" control="" (table="">
4-jadval. Davolanishdan keyingi 28-kuni 200 mg/kg ekstrakti dozasida buyrak toshlarini davolashga nisbatan o'simlik ekstraktlarining sarum fermenti faolligiga ta'siri.Aralash ekstrakt {0}} A. aspera, S. punctata va R. abyssinicus ekstraktlarining kombinatsiyasi (1:1:1 nisbat). Ma'lumotlar har bir guruhdagi 6 ta kalamush uchun o'rtacha ± SD sifatida taqdim etildi (n=6). Vositalar o'rtasidagi taqqoslash I guruh (avtomobilni boshqarish) va II guruh (litiatik nazorat) bilan taqqoslandi. *p < 0.{{10}}5,="" **p="">< 0.01,***p="">< 0.001="" nisbatan="" sezilarli="" oʻzgarishlarni="" koʻrsatadi.="" i="" guruh="" (avtomobilni="" boshqarish),="" #p=""><0,05, ##p=""><0,01, ###p=""><0,001 ii="" guruh="" (litiatik="" nazorat)="" bilan="" solishtirganda="" sezilarli="" o'zgarishlarni="">
Buyrak homogenatini tahlil qilish
Kaltsiy, fosfor va oksalat kontsentratsiyasi litiatik guruhda (II guruh) normal nazoratga (I guruh) nisbatan oshdi. Barcha muolajalar fosfat darajasining sezilarli darajada pasayishini ko'rsatdi (P<0.050), except="" treatment="" with="" buoh="" and="" chl.="" extracts="" of="" g.fruticosus.="" the="" administration="" of="" the="" aqueous="" mixed="" extract="" and="">G. fruticosusEtOAc ekstrakti ishlov berilmagan litiatik guruhlarga nisbatan oksalat darajasini sezilarli darajada kamaytirdi (P).<0.011)(table>
5-jadval. 200 mg/kg dozada 28-kuni davolovchi testdan so'ng o'simlik ekstraktlarining fosfat, oksalat va kaltsiyning buyrak gomogenati tarkibiy qismlariga ta'siri.Aralash ekstrakt {0}} A. aspera, S. punctata va R. abyssinicus ekstraktlarining kombinatsiyasi (1: 1:1 nisbat). Ma'lumotlar har bir guruhdagi 6 ta kalamush uchun o'rtacha ± SD sifatida taqdim etildi (n=6). Vositalar o'rtasidagi taqqoslash I guruh (avtomobilni boshqarish) va II guruh (litiatik nazorat) bilan taqqoslandi. *p < 0.{{10}}5,="" **p="">< 0.01,***p="">< 0.001="" nisbatan="" sezilarli="" oʻzgarishlarni="" koʻrsatadi.="" guruh="" i="" (avtomobil="" nazorati),="" #p=""><0,05, ##p=""><0,01, ###p=""><0,001 ii="" guruh="" (litiatik="" nazorat)="" bilan="" solishtirganda="" sezilarli="" o'zgarishlarni="">
Buyraklarni CaOx kristalli cho'kmalariga nisbatan baholash. Buyrakning gistologik bo'limlarida CaOx kristallarini yorug'lik mikroskopik tekshiruvi natijasida quvurli lümenda CaOx kristallari aniqlandi. CaOx konlarining hajmi va miqdori davolash qilingan guruhlar orasida litiatik nazorat bilan solishtirganda kamaydi. ning ta'siriG. fruticosusEtOAc ekstrakti (G) va uning BuOH fraktsiyasi (H) litiatik nazorat (B) bilan solishtirganda kristallar sonini kamaytirdi, ammo suvli ekstraktlar uchun kamaymaydi.G. fruticosus(I) (2-rasm).
2-rasm. CaOx kristalli cho'ktirishga qarshi ta'sir ko'rsatish uchun o'simlik ekstraktlarida sinovdan o'tgan erkak Wistar kalamushlarining buyrak qismlarining fotomikroskopik (100x) tasviri (ekstraktlar dozasi 200 mg/kg).Buyrak to'qimalarining gistopatologiyasi a Oddiy nazorat/avtomobil, b Litiatik nazorat, c K-Cit, d Cystone, e.G. fruticosusPET ekstrakti, fG. fruticosusChl. ekstrakti, gG. frutikozEtOAc ekstrakti, hG. fruticosusBuOH ekstrakti va iG. fruticosusaq. kasr. Aralash ekstraktlar (1:1:1 nisbatda A. aspera, S. punctata va R. abyssinicus ekstraktlari birikmasi). Buyrak kanalchalarida polimorf tartibsiz CaOx kristallari (sariq doira/strelkalar). Tasvirlar gematoksilin-eozin bo'yalgan 5 mkm qalinlikdagi kerosin bo'laklari edi.
cistanche tubulosa ekstrakti foydasi: aniq erkin radikallar
G.fruticosus ekstraktining buyraklardagi CaOx cho'kishiga ta'siri
Kristal konlari soni korteks, medulla va papilladan 4 ta mikroskopik maydonning o'rtacha qiymatini hisobga olgan holda hisoblangan. Litogen guruhlar bilan solishtirganda, buyraklardagi CaOx kristalli konlari sezilarli darajada kamaydi.G.fruticosusEtOAc ekstrakti p<0.010). the="" number="" of="" crystal="" deposits="" was="" counted="" via="" a="" sagittal="" section="" or="" longitudinal="" plane="" of="" each="" renal="" tissue="" specimen="" divided="" into="" 4="" equal-sized="" regions(two="" virtual="" lines)and="" taking="" the="" mean="" of="" all="" fields(fig.="" 3).="" it="" has="" been="" noted="" that="">G. fruticosusEtOAc ekstrakti urolitiyozni davolashda eng kuchli vosita edi, undan keyin BuOH ekstrakti.
3-rasm. G. fruticosusning turli xil ketma-ket ekstraktlari (ekstraktlar dozasi: 200 mg/kg) bilan davolashdan so'ng shifobaxsh tadqiqotda CaOx kristalining cho'kishi ko'rsatkichlari.Ma'lumotlar har bir guruh uchun n ta {0}} kalamushning o'rtacha ± SD sifatida ifodalangan. *p < 0.05,="" **p="">< 0,01="" va="" ***p="">< 0,001="" litiatik="" nazorat="" (giperoksalurik="" guruh)="" bilan="" solishtirganda="" sezilarli="" o'zgarishlarni="">
cistanche tubulosa ekstrakti foydasi: bakteriyaga qarshi
Munozara
Buyraklar qondan chiqindi mahsulotlarni filtrlaydi va ularni siydikga chiqaradi, agar chiqindi moddalar siydikda to'liq erimasa, buyrak toshlari paydo bo'lishiga olib keladi [59]. Biomineralizatsiya jarayonlari buyrak kanalchalarida supersaturatsiya, yadrolanish, o'sish, agregatsiya va ushlab turish kabi ketma-ket fizik-kimyoviy o'zgarishlarni o'z ichiga oladi [60,61]. Giperoksaluriya giperkaltsiuriyaga qaraganda buyrak toshlarining paydo bo'lishining asosiy xavf omilidir [62].
Siydikda oksalat, fosfat va kaltsiyning chiqarilishi ko'paydi, siydikda magniy va sitrat darajasi 200 mg / kg bw dozasida davolangan guruhga nisbatan kamaydi, bu Lemann va boshqalar tomonidan bildirilgan tadqiqotlarga o'xshash edi. [63] va Sathish va boshqalar. [39]. Siydikdagi mineral tarkibiy qismlarning ko'payishi buyrak naychalari reabsorbtsiyasining buzilishi bilan bog'liq bo'lishi mumkin. Xuddi shunday, elektrolitlar (natriy, kaliy va xlorid) buyrak toshlarining shakllanishini osonlashtiradigan buyrak atsidozini tekshirish uchun ishlatiladi [64]. Davolovchi tadqiqotda natriy darajasi sezilarli darajada kamaydiG. fruticosusEtOAc ekstrakti (P<0.001).in contrast,="" previous="" studies="" reported="" that="" excessive="" excretions="" of="" sodium="" and="" chloride="" in="" the="" urine="" indicate="" the="" diuretic="" activity="" of="" the="" test="" extract="" [65,="" 66],="" with="" increasing="" urinary="" output,="" which="" inhibits="" stone="" developments="">
Magniy siydikda eruvchan oksalat komplekslarini hosil qilish uchun oksalat ionlari bilan bog'lanadi va CaOx hosil bo'lishiga olib keladigan kaltsiy bilan bog'langan oksalatning mavjudligini kamaytiradi [68,69]. Shunday qilib, CaOx toshlari ko'pincha magniy etishmovchiligi bo'lgan odamlar tomonidan hosil bo'ladi, deb taxmin qilish mumkin. Bu haqiqat tomonidan qo'llab-quvvatlandiG. fruticosusEtOAc ekstrakti magniy va sitrat darajasini oshirdi (P<0.010) similar="" to="" k-cit="" in="" the="" curative="" test.="" the="" etoac="" extract="" of="">G. fruticosussiydikda oksalat miqdorining pasayishi (P<0.001) compared="" to="" lithiatic="" control="" in="" the="" curative="" study.="" in="" the="" curative="" test,="" a=""><0.01) reduction="" in="" urinary="" levels="" of="" calcium="" and="" phosphate="" following="" g.fruticosus="" buoh="" extract="" administration="" indicates="" the="" presence="" of="" caox="" inhibitory="" constituents,="" which="" interfere="" with="" crystal="" nucleation="" and="" aggregations.="" moreover,="" an="" increase="" in="" calcium="" concentrations="" is="" a="" favoring="" environment="" for="" nucleation,="" and="" precipitation="" of="" calcium="" oxalate="" or="" calcium="" phosphate="" with="" subsequent="" crystal="" growth="" [32,39].="" similarly,="" increased="" urinary="" phosphate="" and="" oxalate="" excretions="" also="" provide="" a="" favorable="" environment="" for="" the="" formation="" of="" calcium="" phosphate,="" in="" turn,="" leading="" to="" calcium="" oxalate="" crystal="" depositions="" in="" the="" renal="" tubules="" [18,="">
Davolash tadqiqotida,G. fruticosusEtOAc ekstrakti (P<0.010) reduced="" serum="" levels="" of="" potassium.="" in="" addition,="" the="" effects="" of="">G. fruticosusXlorid darajasi bo'yicha BuOH ekstrakti shifobaxsh tadqiqot davomida normal nazoratga yaqin ekanligi aniqlandi. Qon zardobidagi natriy darajasi sezilarli darajada kamaydiG. fruticosusBuOH ekstraktlari (P<0.050) compared="" to="" lithiatic="" control="" in="" the="" curative="" study.="" there="" was="" a="" decrease="" in="" serum="" oxalate="" excretions="" by="" etoac="" and="" buoh="" extracts="" of="">G.fruticosuslitiatik nazorat bilan solishtirganda. Bu oksalat hosil bo'lishining tormozlanishi yoki oksalat metabolizmining buzilishi bilan bog'liq bo'lishi mumkin. Xuddi shunday,G. fruticosusEtOAc va BuOH ekstraktlari (p<0.050) decreased="" calcium="" levels="" compared="" to="" lithiatic="" control="" in="" the="" curative="" test.="" the="" ability="" of="" these="" extracts="" to="" alter="" calcium="" and="" oxalate="" excretions="" may="" be="" due="" to="" the="" disintegration="" of="" mucoproteins,="" which="" are="" promoters="" of="" crystallization="" as="" reported="" by="" doddola="" et="" al.="" [34].="" in="" the="" present="" study,="" the="" level="" of="" citrate="" in="" the="" serum="" was="" increased="" by="">G. fruticosusBuOH ekstraktlari (P<0.011), suggesting="" a="" possible="" curative="" effect="" close="" to="" k-cit="" treated="" group=""><0.001). the="" presence="" of="" tannins="" and="" flavonoids="" can="" lead="" to="" the="" relaxation="" of="" smooth="" muscles="" of="" the="" urinary="" tract,="" which="" could="" facilitate="" the="" expulsion="" of="" kidney="" stones="" as="" studied="" in="" rats="" [54],="" which="" were="" confirmed="" their="" presence="" in="" the="" phytochemical="" analysis="" of="">G. fruticosusushbu tadqiqotdagi ekstraktlar. Hozirgi terapevtik tadqiqotda ham aniq isbotlanganki, G. frutikozBuOH ekstraktlari AST darajasini sezilarli darajada pasaytirdi (P<0.001), which="" was="" close="" to="" k-cit="" compared="" to="" the="" lithiatic="" control="" in="" the="" curative="" studies.="" this="" can="" be="" attributed="" to="" the="" fact="" that="" abnormal="" levels="" of="" liver="" enzymes,="" particularly="" aminotransferases="" including="" alanine="" aminotransferase(alt),="" and="" aspartate="" aminotransferase="" (ast)="" are="" prognostic="" features="" (indicators)of="" the="" damages="" of="" liver="" cells,="" and="" the="" cellular="" integrity="" of="" the="" kidneys="" [71,="">
Buyraklardagi eksperimental CaOx kristalining cho'kishi ham mahalliy yallig'lanish bilan bog'liq, bu monotsitlar va makrofaglarning saytga infiltratsiyasidan dalolat beradi [73]. G.fruticosus EtOAc ekstrakti (P<0.051)and its="" buoh="" extract=""><0.051) treated="" groups,="" the="" severity="" of="" caox="" crystal="" depositions="" reduced="" the="" kidneys="" compared="" to="" lithiatic="" control,="" which="" was="" similar="" to="" a="" study="" on="" the="" other="" herbal="" extract="" [74].="" in="" the="" curative="" study,="" etoac="" extract="" of="">G.fruticosusfosfat darajasini sezilarli darajada kamaytirdi (P<0.051)compared to="" the="" lithiatic="" control.="" tissue="" injury="" could="" be="" caused="" by="" exposures="" to="" phosphate="" and="" calcium="" phosphate="" crystals,="" leading="" to="" the="" generation="" of="" oxidative="" stress,="" lipid="" peroxidation,="" and="" depletion="" of="" antioxidant="" enzymes="" [75].="" consequently,="" the="" renal="" epithelial="" injury="" promotes="" crystal="" retention,="" as="" epithelial="" injury="" exposes="" a="" variety="" of="" crystal="" adhesion="" molecules="" on="" epithelial="" surfaces="" and="" promotes="" stone="" formation="" [73,="" 76].="" com="" accumulates="" in="" the="" kidneys="" by="" attaching="" to="" tubular="" cell="" membranes,="" followed="" by="" internalization="" by="" endocytosis,="" leading="" to="" cell="" death="" [75].="" the="" precipitation="" of="" oxalate="" as="" com="" in="" the="" tubular="" lumen="" has="" been="" linked="" with="" renal="" toxicity="" and="" inflammation,="" damaging="" the="" structures="" of="" mitochondria,="" inhibiting="" mitochondrial="" respiratory="" functions="" in="" proximal="" tubular="" cells,="" altering="" cellular="" permeability,="" and="" leading="" to="" renal="" cell="" death="">
Tadqiqotning asosiy cheklovi - bu ma'lumotlar sifatini yaxshilaydigan COM va COD kristallarini farqlash uchun in vivo kristallanishlarni kattalashtiradigan va yaxshiroq qayta hal qiladigan qutblangan yorug'lik mikroskopidan foydalanishning mumkin emasligi. Sinov ekstraktining siydik diuretik faolligiga ta'siri baholanmagan. Ushbu cheklovlarga qaramay, ushbu tadqiqotning kuchi tadqiqotda ishlatiladigan an'anaviy dorivor o'simliklarning potentsial samaradorligini tekshirish uchun standart eksperimental dizayn va protseduralardan foydalanish edi.
Cistanche yallig'lanishga qarshi bo'lishi mumkin
Xulosa
Ushbu tadqiqot an'anaviy foydalanish uchun ba'zi dalillarni taqdim etdiG. fruticosusurolitiyozni davolashda ekstraktlar. In vivo/hayvon sinovlari shifobaxsh samaradorligini tasdiqladiG. fruticosusbiokimyoviy va gistopatologik topilmalar bilan tasdiqlangan ekstraktlar. Ushbu topilmalar CaOx ning shifobaxsh samaradorligini ko'rsatdiG. fruticosusEtOAc ekstrakti. Urolitiyozni davolash uchun mas'ul bo'lgan faol birikmalarni aniqlash uchun qo'shimcha tadqiqotlar o'tkazish kerak.
Ma'lumotnomalar
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